QIAGEN RNA EXTRACTION KIT HANDBOOK



Qiagen Rna Extraction Kit Handbook

RNeasy Mini Kit from Qiagen Biocompare Product Review. The QIAsymphony PAXgene Blood RNA Kit is intended for purification of intracellular RNA, including miRNA, from human whole blood (4.8 x 106–1.1 x 107 leukocytes/ml). The QIAsymphony PAXgene Blood RNA Kit is not for the isolation of genomic DNA or viral nucleic acids from human whole blood., The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g., DNase digestion, proteinase digestion, RNA ligation, and labeling reaction).

RNeasy Plant Mini Kit from Qiagen Biocompare Product Review

QIAamp Viral RNA Mini Handbook MolecularLab.it. 12 RNeasy Mini Handbook 06/2001. The RNAlater principle and procedure. RNAlater RNA Stabilization Reagent, included in RNeasy Protect Kits, represents a novel technology for RNA protection in animal tissues and other biological samples, allowing transport and storage at ambient temperatures., DNeasy 96 Blood & Tissue Kit For purification of total DNA from animal blood animal tissue rodent tails ear punches cultured cells DNeasy Blood & Tissue Handbook 07/2006 5 Kit Contents DNeasy Blood & Tissue Kit (50) (250) Catalog no. 69504 69506 for each QIAGEN kit and kit component..

miRNeasy Mini Handbook 10/2007 9 miRNA purification in 96 wells, from FFPE tissues, and from cells The miRNeasy Mini Kit enables low-throughput RNA purification using spin columns. For high-throughput purification in a 96-well format, the miRNeasy 96 Kit is available. Total RNA and miRNA can also be copurified from formalin-fixed, paraffin-embedded 10 RNeasy MinElute Cleanup Handbook 03/2003 Protocol: RNA Cleanup This protocol is designed to clean up RNA from enzymatic reactions, for desalting RNA samples, and for concentration of RNA isolated by various methods. For concentration of total cellular RNA purified using the PAXgene Blood RNA Kit, see the RNA cleanup protocol on page 13.

Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software. RNeasy Mini Handbook 04/2006 9 Introduction The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic

The RNeasy Protect Mini Kit is supplied with RNA later RNA Stabilization Reagent, which represents a novel technology for the immediate preservation of the gene expression pattern in animal tissues, enabling reliable gene expression analysis. RNeasy Mini Handbook 04/2006 9 Introduction The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic

The PAXgene Blood RNA Kit is for the purification of intracellular RNA from whole blood collected in the PAXgene Blood RNA Tube. When the kit is used in conjunction with the PAXgene Blood RNA Tube , the system provides purified intracellular RNA from whole blood for RT-PCR used in … Remove RNAlater®-stabilized tissue from the reagent using forceps. For RNeasy Protect Mini Kit (cat. nos. 74124 and 74126), please start with the Quick-StartProtocol RNAlater RNA Stabilization Reagent, RNAlater TissueProtect Tubes, and RNeasy Protect Kits. * This option not included for cells in handbook; handbook to be updated. 1. Cells

In the past 5 years alone, over 100,000 scientific publications have cited QIAGEN purification kits. That’s because our tools provide high purity and yield nucleic acids time and again. So for your next experiment, trust our gold standard purification technologies, and obtain the high-quality results you need. 12 RNeasy Mini Handbook 06/2001. The RNAlater principle and procedure. RNAlater RNA Stabilization Reagent, included in RNeasy Protect Kits, represents a novel technology for RNA protection in animal tissues and other biological samples, allowing transport and storage at ambient temperatures.

The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g., DNase digestion, proteinase digestion, RNA ligation, and labeling reaction) The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes.

The AllPrep DNA/RNA/Protein Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. All due care and attention should be exercised in the handling of the product. This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively. This …

RNeasy Lipid Tissue Handbook ResearchGate

Qiagen rna extraction kit handbook

RNeasy Mini Kit from Qiagen Biocompare Product Review. Jan 22, 2018 · Using built-in gDNA eliminator technology, the RNeasy Plus Kit gives you high yields of high-integrity RNA in minutes for any downstream application, without the …, Jun 25, 2009 · Qiagen’s RNeasy Plant Mini RNA Extraction Kit is a widely used and generally robust kit which yields high quality RNA from a multitude of plant samples. It is the kit of choice in our lab although we have tried at least three in-house protocols and two other kits from Peqlab and Invitrogen..

RNeasy Mini Handbook IHES. The RNeasy Protect Mini Kit is supplied with RNA later RNA Stabilization Reagent, which represents a novel technology for the immediate preservation of the gene expression pattern in animal tissues, enabling reliable gene expression analysis., 6 QIAGEN OneStep RT-PCR Kit Handbook 05/2002 Introduction The QIAGEN OneStep RT-PCR Kit provides a convenient format for highly efficient and specific RT-PCR using any RNA. The kit contains optimized components that allow both reverse transcription and PCR amplification to take place in what is commonly referred to as a “one-step” reaction..

QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit

Qiagen rna extraction kit handbook

QIAamp Viral RNA Mini Handbook MolecularLab.it. RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi. https://en.wikipedia.org/wiki/QGEN_(NASDAQ) Get high-quality RNA fast, free of lingering PCR inhibitors with the RNeasy PowerMicrobiome Kit. Designed for purification of total RNA from stool, dried feces, gut material, contaminated buccal swabs and secretions, the RNeasy Power Microbiome Kit isolates RNA ….

Qiagen rna extraction kit handbook


QIAquick® Gel Extraction Kit The QIAquick Gel Extraction Kit (cat. nos. 28704 and 28706) can be stored at room temperature (15–25°C) for up to 12 month. For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi.

8 RNeasy Midi/Maxi Handbook 06/2001 Introduction RNA isolation RNeasy Midi and Maxi Kits are designed for rapid isolation of up to 1 mg or 6 mg of total RNA, respectively, from cells and tissues, bacteria, and yeast. RNeasy Protect Midi and Maxi Kits provide stabilization of RNA in animal cells and tissues and purification of In the past 5 years alone, over 100,000 scientific publications have cited QIAGEN purification kits. That’s because our tools provide high purity and yield nucleic acids time and again. So for your next experiment, trust our gold standard purification technologies, and obtain the high-quality results you need.

Innovation by QIAGEN creates sample and assay technologies that enable insights — making improvements in life possible. Download apps for your mobile devices, or use our web apps to access interactive protocols or dive deep inside a cell to explore it's RNA. The QIAamp DNA Blood Mini Kit is designed for processing up to 200 μl fresh or frozen human whole blood. QIAamp Mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Purification of DNA using the QIAamp DNA Blood Mini Kit can be fully automated on the QIAcube …

RNeasy Micro Handbook 04/2003 5 All due care and attention should be exercised in the handling of the products. We recommend all users of QIAGENВ® products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines. The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 Ојg RNA. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

The PAXgene Blood RNA Kit is for the purification of intracellular RNA from whole blood collected in the PAXgene Blood RNA Tube. When the kit is used in conjunction with the PAXgene Blood RNA Tube , the system provides purified intracellular RNA from whole blood for RT-PCR used in … The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.

Apr 06, 2010В В· The RNeasy Mini Kit for isolation of total RNA from Qiagen is a very convenient kit for isolation of good quality RNA in a relatively short time. The kit comes with all the required reagents for the purification including the columns, collection tubes, buffers and even RNase-free water. QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Our advanced, high-quality products and services ensure success from sample to result. QIAGEN sets standards in: Purification of DNA, RNA, and proteins

Qiagen rna extraction kit handbook

The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut … For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.QIAGEN.com or can be requested from QIAGEN Technical Services or your local distributor.

RNeasy Plus Kit High performance RNA extraction YouTube

Qiagen rna extraction kit handbook

RNeasy® Mini Handbook Third Edition. This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively. This …, RNeasy Mini Handbook 04/2006 9 Introduction The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic.

QIAGEN Reproducibility

RNeasy Mini Handbook IHES. The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system., The QIAsymphony PAXgene Blood RNA Kit is intended for purification of intracellular RNA, including miRNA, from human whole blood (4.8 x 106–1.1 x 107 leukocytes/ml). The QIAsymphony PAXgene Blood RNA Kit is not for the isolation of genomic DNA or viral nucleic acids from human whole blood..

The PAXgene Blood RNA Kit is for the purification of intracellular RNA from whole blood collected in the PAXgene Blood RNA Tube. When the kit is used in conjunction with the PAXgene Blood RNA Tube , the system provides purified intracellular RNA from whole blood for RT-PCR used in … QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Our advanced, high-quality products and services ensure success from sample to result. QIAGEN sets standards in: Purification of DNA, RNA, and proteins

RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column. the QIAamp MinElute Virus Spin Kit for purification of high-quality viral nucleic acids. The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. The range of protocols available is continually expanding, and additional QIAGEN protocols can be

RNeasy Mini Handbook 04/2006 9 Introduction The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic QIAGEN Plasmid Purification Handbook 11/2005 19. Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits. This protocol is designed for preparation of up to 100 µg of high- or low-copy plasmid or cosmid DNA using the QIAGEN Plasmid Midi Kit, or up to 500 µg using the QIAGEN Plasmid Maxi Kit.

Sep 25, 2007В В· The RNeasy Mini Kit by Qiagen is used to purify RNA from animal cells or tissues, yeast and bacteria. It comes with RNeasy mini spin columns, collection tubes and RNase-free reagents and buffers. It also comes... The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 Ојg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 Ојg) can be achieved using the High-Yield Supplementary Protocol.

Remove RNAlaterВ®-stabilized tissue from the reagent using forceps. For RNeasy Protect Mini Kit (cat. nos. 74124 and 74126), please start with the Quick-StartProtocol RNAlater RNA Stabilization Reagent, RNAlater TissueProtect Tubes, and RNeasy Protect Kits. * This option not included for cells in handbook; handbook to be updated. 1. Cells Sep 25, 2007В В· The RNeasy Mini Kit by Qiagen is used to purify RNA from animal cells or tissues, yeast and bacteria. It comes with RNeasy mini spin columns, collection tubes and RNase-free reagents and buffers. It also comes...

RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column. The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 Ојg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 Ојg) can be achieved using the High-Yield Supplementary Protocol.

QIAamp DNA Blood Mini Kits, or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or special-ized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any The AllPrep DNA/RNA/Protein Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. All due care and attention should be exercised in the handling of the product.

This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively. This … RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column.

6 QIAamp Viral RNA Mini Handbook 12/2007 Quality Control In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of QIAamp Viral RNA Mini Kits is tested against predetermined specifications to ensure consistent product quality. Safety Information When working with chemicals, always wear a suitable lab coat, disposable Jan 22, 2018 · Using built-in gDNA eliminator technology, the RNeasy Plus Kit gives you high yields of high-integrity RNA in minutes for any downstream application, without the …

QIAamp® DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook For DNA purification from Whole blood Plasma Serum Buffy coat Body fluids Purification of viral RNA and DNA 9 Purification of DNA from urine 9 QIAamp DNA Blood Mini Kits, or QIAGEN products in general, please do not hesitate In the past 5 years alone, over 100,000 scientific publications have cited QIAGEN purification kits. That’s because our tools provide high purity and yield nucleic acids time and again. So for your next experiment, trust our gold standard purification technologies, and obtain the high-quality results you need.

The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut … Jun 25, 2009 · Qiagen’s RNeasy Plant Mini RNA Extraction Kit is a widely used and generally robust kit which yields high quality RNA from a multitude of plant samples. It is the kit of choice in our lab although we have tried at least three in-house protocols and two other kits from Peqlab and Invitrogen.

Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software. Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.

8 RNeasy Midi/Maxi Handbook 06/2001 Introduction RNA isolation RNeasy Midi and Maxi Kits are designed for rapid isolation of up to 1 mg or 6 mg of total RNA, respectively, from cells and tissues, bacteria, and yeast. RNeasy Protect Midi and Maxi Kits provide stabilization of RNA in animal cells and tissues and purification of RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi.

The AllPrep DNA/RNA/Protein Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. All due care and attention should be exercised in the handling of the product. For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.QIAGEN.com or can be requested from QIAGEN Technical Services or your local distributor.

QIAamp DNA Blood Mini Kits, or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or special-ized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any The PAXgene Blood RNA Kit is for the purification of intracellular RNA from whole blood collected in the PAXgene Blood RNA Tube. When the kit is used in conjunction with the PAXgene Blood RNA Tube , the system provides purified intracellular RNA from whole blood for RT-PCR used in …

QIAamp DNA Investigator Kit QIAGEN

Qiagen rna extraction kit handbook

Resources QIAGEN. Remove RNAlaterВ®-stabilized tissue from the reagent using forceps. For RNeasy Protect Mini Kit (cat. nos. 74124 and 74126), please start with the Quick-StartProtocol RNAlater RNA Stabilization Reagent, RNAlater TissueProtect Tubes, and RNeasy Protect Kits. * This option not included for cells in handbook; handbook to be updated. 1. Cells, The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes..

QIAsymphony PAXgene Blood RNA Kit Handbook. QIAampВ® DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook For DNA purification from Whole blood Plasma Serum Buffy coat Body fluids Purification of viral RNA and DNA 9 Purification of DNA from urine 9 QIAamp DNA Blood Mini Kits, or QIAGEN products in general, please do not hesitate, RNeasyВ® Fibrous Tissue Handbook RNeasy Fibrous Tissue Mini Kit RNeasy Fibrous Tissue Midi Kit For purification of total RNA from heart, skeletal muscle, aorta, and other fiber-rich tissues November 2006 WWW.QIAGEN.COM.

QIAamp DNAMiniand BloodMiniHandbook

Qiagen rna extraction kit handbook

DNeasy Blood & Tissue Handbook. In the past 5 years alone, over 100,000 scientific publications have cited QIAGEN purification kits. That’s because our tools provide high purity and yield nucleic acids time and again. So for your next experiment, trust our gold standard purification technologies, and obtain the high-quality results you need. https://en.wikipedia.org/wiki/QGEN_(NASDAQ) The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g., DNase digestion, proteinase digestion, RNA ligation, and labeling reaction).

Qiagen rna extraction kit handbook

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  • RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column. the QIAamp MinElute Virus Spin Kit for purification of high-quality viral nucleic acids. The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. The range of protocols available is continually expanding, and additional QIAGEN protocols can be

    Sep 25, 2007В В· The RNeasy Mini Kit by Qiagen is used to purify RNA from animal cells or tissues, yeast and bacteria. It comes with RNeasy mini spin columns, collection tubes and RNase-free reagents and buffers. It also comes... RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi.

    RNeasy Mini Handbook 04/2006 9 Introduction The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic Remove RNAlater®-stabilized tissue from the reagent using forceps. For RNeasy Protect Mini Kit (cat. nos. 74124 and 74126), please start with the Quick-StartProtocol RNAlater RNA Stabilization Reagent, RNAlater TissueProtect Tubes, and RNeasy Protect Kits. * This option not included for cells in handbook; handbook to be updated. 1. Cells

    The QIAsymphony PAXgene Blood RNA Kit is intended for purification of intracellular RNA, including miRNA, from human whole blood (4.8 x 106–1.1 x 107 leukocytes/ml). The QIAsymphony PAXgene Blood RNA Kit is not for the isolation of genomic DNA or viral nucleic acids from human whole blood. The PAXgene Blood RNA Kit is for the purification of intracellular RNA from whole blood collected in the PAXgene Blood RNA Tube. When the kit is used in conjunction with the PAXgene Blood RNA Tube , the system provides purified intracellular RNA from whole blood for RT-PCR used in …

    QIAampВ® DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook For DNA purification from Whole blood Plasma Serum Buffy coat Body fluids Purification of viral RNA and DNA 9 Purification of DNA from urine 9 QIAamp DNA Blood Mini Kits, or QIAGEN products in general, please do not hesitate The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 Ојg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 Ојg) can be achieved using the High-Yield Supplementary Protocol.

    8 RNeasy Midi/Maxi Handbook 06/2001 Introduction RNA isolation RNeasy Midi and Maxi Kits are designed for rapid isolation of up to 1 mg or 6 mg of total RNA, respectively, from cells and tissues, bacteria, and yeast. RNeasy Protect Midi and Maxi Kits provide stabilization of RNA in animal cells and tissues and purification of Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.

    The EZ1 DNA Investigator Kit reproducibly automates purification of genomic DNA from reference and casework samples in human identity testing. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), EZ1 Advanced (1–6 samples per run), and … For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.QIAGEN.com or can be requested from QIAGEN Technical Services or your local distributor.

    The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Our advanced, high-quality products and services ensure success from sample to result. QIAGEN sets standards in: Purification of DNA, RNA, and proteins

    miRNeasy Mini Handbook ForpurificationoftotalRNA,including miRNA,fromanimalandhumancells andtissues commercial RNA purification kits do not recover RNA molecules smaller than ~200 nucleotides. The miRNeasy Mini Kit is designed for purification of total RNA, miRNeasy Mini Kit QIAGEN 8 DNeasy Tissue Handbook 03/2004 Introduction DNeasy Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and viral) from a variety of sample sources including fresh or frozen animal tissues and cells, yeasts, or bacteria. DNeasy purified DNA is free of contaminants and

    6 QIAamp Viral RNA Mini Handbook 12/2007 Quality Control In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of QIAamp Viral RNA Mini Kits is tested against predetermined specifications to ensure consistent product quality. Safety Information When working with chemicals, always wear a suitable lab coat, disposable Remove RNAlater®-stabilized tissue from the reagent using forceps. For RNeasy Protect Mini Kit (cat. nos. 74124 and 74126), please start with the Quick-StartProtocol RNAlater RNA Stabilization Reagent, RNAlater TissueProtect Tubes, and RNeasy Protect Kits. * This option not included for cells in handbook; handbook to be updated. 1. Cells

    QIAquick® Gel Extraction Kit The QIAquick Gel Extraction Kit (cat. nos. 28704 and 28706) can be stored at room temperature (15–25°C) for up to 12 month. For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. The RNeasy Mini Handbookprovides protocols for use with the following kits: RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g., DNase digestion, proteinase digestion, RNA ligation, and labeling reaction)

    RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column. Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.

    RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi. RNeasy Plus Mini Handbook 10/2005 7 Introduction The RNeasy Plus Mini Kit is designed to purify RNA from small amounts of animal cells or tissues. The kit is compatible with a wide range of cultured cells and with easy-to-lyse tissues. Genomic DNA contamination is effectively removed using a specially designed gDNA Eliminator spin column.

    The QIAsymphony PAXgene Blood RNA Kit is intended for purification of intracellular RNA, including miRNA, from human whole blood (4.8 x 106–1.1 x 107 leukocytes/ml). The QIAsymphony PAXgene Blood RNA Kit is not for the isolation of genomic DNA or viral nucleic acids from human whole blood. miRNeasy Mini Handbook 10/2007 9 miRNA purification in 96 wells, from FFPE tissues, and from cells The miRNeasy Mini Kit enables low-throughput RNA purification using spin columns. For high-throughput purification in a 96-well format, the miRNeasy 96 Kit is available. Total RNA and miRNA can also be copurified from formalin-fixed, paraffin-embedded

    Sep 25, 2007 · The RNeasy Mini Kit by Qiagen is used to purify RNA from animal cells or tissues, yeast and bacteria. It comes with RNeasy mini spin columns, collection tubes and RNase-free reagents and buffers. It also comes... The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut …